Equipment

The Shared-Access Equipment Centre “Industrial Biotechnology” of Federal Research Center “Fundamentals of Biotechnology” Russian Academy of Sciences (A.N. Bach Institute of biochemistry RAS) has a park of modern hi-tech equipment for different researches in the field of genomic and postgenomictechnologies, bioengineering, systembiology, as well as synthetic and structural biology.

For viewing the data about equipment anf technical characteristics please choose one the department.

  • Chromatographic department

    Name Model Manufacturer Country of origin Year of release
    1 Gas chromatograph with MS detector GC-MS QP 2010 Ultra EI Shimadzu Japan 2015
     2 Gas chromatograph with FID, ECD  Crystall 5000.2 FID-ECD  Chromatec  Russia 2008
     3 Capillary electrophoresis system with PDA-detector  P/ACE MDQ  Beckman Coulter  USA 2010
     4 HPLC-UV with autosampler  Smartline  Knauer  Germany 2007
     5  HPLC with MS detector LC-QQQ  Agilent 6460  Agilent Technologies  USA 2014
     6 HPLC with fluorescense and refractometer detectors  Stayer Aquilon Russia 2005
     7 XRF-spectormeter  Renom-FV  Renom Russia 2009
    Name Technical characteristics
    1 Gas chromatograph with MS detector
    Tap for technical characteristics

    Range of masses: To 1,5-1024 a.a.
    Range of the thermostat of columns: from 40 to 450 °C
    Ratio signal/noise: not less than 60

     2 Gas chromatograph with FID, ECD  
    Tap for technical characteristics

    Column thermstate: from room temp+4 °С to 450 °С
    Detector temperature limit not more than 450 °C
    FID detection limit:1,4*10-12 gС/s
    ECD detection limit: 2*10-14 g/s

     3 Capillary electrophoresis system with PDA-detector  
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    Range of voltage: Bipolar 1 ÷ 30 kV (1÷10 of kV for injection) with a step of 0,1 kV
    Current range: 3-300 mkA
    Range of pressure:
    −34 ÷ 690 kPas (washing / division)
    −172 ÷ 690 kPas (injection)
    Sample temperature: 5 °C ÷ 60 °C
    Accuracy of installation of the wavelength of 1 nanometer

     4 HPLC-UV with autosampler   
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    Spectral range: 190-600 nanometers
    Range of installation of a total flow: 0,01-19,98, ml/min.
    Range of pressure: 0-30 MPa
    Reproducibility of formation of a gradient: not less than 0,4%
    Accuracy of indications in a linear interval of pressure, not less than 2%

     5 HPLC with MS detector LC-QQQ   
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    Mass range 5-3000 Da
    Scan rate12500 Da/s

     6 HPLC with fluorescense and refractometer detectors   
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    Refractometer:
    Detection limit (glucose): < 1·10-5 g
    Wavelength: 650 nm
    Fluorescende:
    Wavelength range: 200 – 650 nm
    Accuracy of wavelength 2 нм

     7 XRF-spectormeter   
    Tap for technical characteristics

    Permission: not worse than 140 eV on the line of 5,9 KEV
    Be window: not more than 7,6 microns
    Maximum speed of the account: to 1 000 000 imp / sec.
    Instrument error: ±0,3%

    Invalid Displayed Gallery

  • Name Model Manufacturer Country of origin Year of release
    1  MALDI-TOF/TOF UltrafleXtreme Bruker Germany 2011
    Name Technical characteristics
    1 MALDI-TOF/TOF
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    Range of masses, to an a.a.
    — in the linear mode – 20-260000
    — in the reflective mode – 20-20000
    Limit of an admissible relative error of measurement of weight, ppm
    — in the linear mode – 50-100
    — in the reflective mode – 10-50
    Permission on a half-height
    — in the linear mode – 5-103 for masses to less than 3-103 a.a., 103 for masses to more than 104 a.a.
    — in the reflective mode – 2,5-104 for masses to less than 3-103 a.a., 103 for masses to more than 104 a.a.
    Detection limit at summation of 50 laser impulses for 1 фмоля at m/z = 2465,2 (ACTH 18-39 peptide), the relation signal/noise – 10
    Stability of a scale of masses:
    deviation within 0,5 hours δm/m, ppm – ±25

  • Name Model Manufacturer Country of origin Year of release
    1 Chirascan™ Circular Dichroism Spectrometer Сhirascan Applied Photophysics United Kindom 2007
    2 UV-Spectrometer with thermomodule Cary-300 Cary Australia 2006
    3 Spectrofluorimeter FluoroMax 4 Horiba Japan 2015
    Name Technical characteristics
    1 Chirascan™ Circular Dichroism Spectrometer
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    Monochromator Dual polarising, dual dispersing monochromator
    Light Source 150W air-cooled Xe arc lamp
    Wavelength range <170nm to 900nm; extended to 1250nm with optional IR detector
    Cell carriage Single cell, Peltier temperature controlled
    Optional 4-cell auto changer also available Detection modes (5 channels) Simultaneous CD, absorbance, temperature, detector HT and DC voltage
    Typical RMS noise specifications (measured at 1nm Bandwidth and 2 sec D.I.T.)
    0.07mdeg @ 175nm 0.03mdeg @ 180nm 0.03mdeg @ 200nm 0.03mdeg @ 250nm 0.04mdeg @ 500nm
    Stray light <3ppm at 200nm
    Wavelength resolution 0.1nm at all wavelengths
    Nitrogen purge requirements 5 l/min across the entire wavelength range; 2 l/min in the near UV (> 200nm)
    Bench space, W x H x D 150 x 55 x 60cm

    2 UV-Spectrometer with thermomodule
    Tap for technical characteristics

    Permission: ≤ 0.24 nanometer
    Photometric accuracy: +/-0.001 And
    Photometric range: 0.001-5 And
    Spectral range: 190–900 nanometers
    Wavelength accuracy: ± 0.2 nanometers
    Peltye thermosystem: 5-90 °C

    3 Spectrofluorimeter
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    150 watt. Without ozon-xenon lamp
    Monochromator excitation:  250 — 850 nm
    Monochromator radiation: 250 — 850 nm
    Correction of spectral sensitivity: 300 — 800 nm
    The spectral slit width: 0.1 — 30 nm
    Length accuracy won: +/- 0.5 nm
    Relationship signal/noise: 3000
    Storing the data in the format of Origin

  • Name Model Manufacturer Country of origin Year of release
    1 Set of equipment for working with nanoparticles includes:
    High-speed centrifuge Allegra 64R
    Microplate reader Multiskan EX
    Allegra 64R
    Multiskan EX
    Beckman Coulter
    Thermo Labsystems
    USA 2004
    Name Technical characteristics
    1 Set of equipment for working with nanoparticles includes:
    High-speed centrifuge Allegra 64R
    Microplate reader Multiskan EX
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    High-speed centrifuge Allegra 64R:

    Rotation speed – 0 to 30000 rpm;

    Maximal relative acceleration – 64400g;
    Maximal capacity – 4×85 mL;
    Tubes capacity – from 1.5 to 50 mL;
    Temperature setting – from −20 to +40°C;
    Ten acceleration / deceleration modes

    Microplate reader Multiskan EX:
    Spectral range – 400-750 nm.
    Measurement range – 0-2.5 OD at 405 nm.
    Accuracy – ±1% (0-2.0 OD).
    Repeatability – <0,2% (0-2.0 OD).
    Linearity – ±1,5% (0-2.0 OD).
    Measurement speed — 5 seconds per 96-well microplate.

  • Name Model Manufacturer Country of origin Year of release
    1 Set of the equipment for strain construction:
    Fermentation system
    The laboratory fermentations system for the microbiological synthesis of biologically active substances
    Equipment for real-time PCR Biorad
    Refrigerated Centrifuge Avanti
    BioRad MicroPulser Electroporator
    Microbiological control point in the complex
    Gel Documentation System Shap id
    Laboratory Autoclave Sanyo Laboautoclave MLS-2420L
    Sartorius Biostat B plus

    CFX96

    AVANTI J-20 XP
    Micropulser
    Innova, Holten

    DP-001.FDC MLS-2420L

    Sartorius, Biorad, Beckmann, Sunrise, Sanyo, ViberLourmant, Knauer USA, Germany, France, Japan 2006
    Name Technical characteristics
    1 Set of the equipment for strain construction
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    The BIOSTAT® B plus fermentation system:
    — Volume 2 L
    — Digital Controller
    — Graphical user interface with color display and touch screen operation
    — Integrated amplifiers for Temperature, pH, DO, combined Foam | Level amplifier
    — Space for Redox and Turbidity amplifier(single only)
    Application driven integrated gassing system, choice of: microbial culture, O2 Enrichment, Gas Flow Ratio Control, cell culture | dual use, Exclusive Flow, cell culture, Additive Low Flow
    — Agitation System: Speed range 20 up to 2,000 rpm, Maintenance free, Gear-free for quite operation

    The laboratory fermentations system for the microbiological synthesis of biologically active substances:
    Interchangeable culture vessels 2/5 l

    Equipment for real-time PCR Biorad:
    Delivers accurate quantification for up to five targets within large volume reactions.
    Max. ramp rate, 5°C / per second
    Temperature Range, 0-100°C
    Temperature Accuracy, ±0.2 of programmed target at 90°C
    Range of Excitation / Emission Wavelengths — 450 — 730 nm

    Refrigerated Centrifuge Avanti:
    Maximum Speed — 20,000 RPM
    Maximum RCF (g Force) — 51,500 x g
    Temperature Range — 10° to 40°C
    Temperature Accuracy — ± 2° of set temperature
    Rotor JLA-8.1000 capacity – up to 6L

    BioRad MicroPulser Electroporator:
    — optimized programs for commonly studied bacteria and fungi allow rapid program selection
    — Output voltage and pulse duration adjustment
    — Voltage adjustable in 200–3,000 V range with 10 V precision; 5 ms default or 1–4 ms with 0.1 ms precision.

    Microbiological control point in the complex:
    Incubator Shakers: Innova® 40R
    — 15°C below ambient to 80°C, min. 4°C.
    — Accurate and Uniform Temperature Control:± 0.1°C with uniformity of ± 0.25°C at 37°C.
    — Agitation: 25 — 500 rpm, ± 1 rpm.
    Thermo Holten Laminar Air —  Class II Biological Safety (Safe 2020/0.9)
    HEPA filter efficiency – 99,995%
    Int dims (H x W x D), (mm) — 725/650 x 900 x 580

    Gel Documentation System Shap id:
    UV Transilluminator TFP-20C/WL (254 nm/ visible light)

    Laboratory Autoclave Sanyo LaboautoclaveMLS-2420L:
    Sterilisation temperature          — 105 ~ 126 °C
    Effective capacity —  20 litres
    Pressure gauge range —           0 — 0.3 Mpa / 0 -45 psi

  • Name Model Manufacturer Country of origin Year of release
    1 Complex of equipment for the development of fermentation technologies KF-103/4, ST-1, RS-4, KF-104, F-10, F-50, LF-1, C-26 Prointex Russia 2006
    2 System of laboratory fermenters LF-1 Infors HT Multifors France 2015
    3 Centrifuge Avanti JXN-26 Avanti USA 2015
    4 Autoclave vertical ELV 3870 Tuttnauer Israel 2011
    5 Freeze dryer Benchtop 6K ES Benchtop USA 2011
    6 Spray dryer Mini Spray Dryer B-290 Buchi Switzerland 2015
    Name Technical characteristics
    1 Complex of equipment for the development of fermentation technologies
    Tap for technical characteristics

    3-liter fermenters:
    The number of fermenters — 8. The working volume of 0.5-2.0 l Measured parameters: pH, pO2, stirrer speed, temperature, foaming. The cultivation modes: periodic, oxistat,  chemostat. Control: a local controller maintaining the set values of pH, pO2, temperature, rpm of the agitator; the computer registration of all parameters of cultivation. It is possible to grow the bacteria, yeasts, fungi. The number of channels for medium feeding — three.

    Sterilizable fermenter 50-liter:
    The working volume of 30 to 60 liters. Measured parameters: pH, pO2, stirrer speed, temperature, foaming. Control: a local controller with registration of all parameters of cultivation. It is possible to grow the bacteria, yeasts, fungi. The number of channels for medium feeding – three.

    10-liter fermenter:
    The number of fermenters is 2. The working volume of 4,0-8,0 L. Measured parameters: pH, pO2, stirrer speed, temperature, foaming. The cultivation modes: periodic, oxistat, hemostat. Control: a local controller maintaining the set values of pH, pO2, temperature, rpm of the agitator; the computer registration of all parameters of cultivation. It is possible to grow the bacteria, yeasts, fungi. The number of channels for feeding — three.

     2 System of laboratory fermenters  
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    The number of fermenters — 8. The working volume of 0.4 — 1.0 liters Measured parameters: pH, pO2, stirrer speed, temperature, foaming. The cultivation modes: periodic, oxistat, hemostat. Control: a local controller maintaining the set values of pH, pO2, temperature, rpm of the agitator; the computer registration of all parameters of cultivation. It is possible to grow the bacteria, yeasts, fungi. The number of channels for feeding — three.

     3 Centrifuge  
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    Maximum speed — 20 000 rpm (51 500g). The capacity of the rotor JLA-8.1000 up to 6 liters, temperature range: -10 : +40ºC

     4 Autoclave vertical   
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    The working volume of 85 L. Temperature regimes and time of sterilization are set by the user. Temperature range — from 100 to 134 oС

     5 Freeze dryer   
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    Drying is designed for drying samples from the frozen state at low pressure (20-30 microtorr). Capacity by the ice up to the 3 liters. Capacity by water up to 2 liters/day.

     6 Spray dryer   
    Tap for technical characteristics

    Evaporative capacity by water 1 l/h. Air flow up to 35 m3/h Max inlet temperature 220 ºC. Range of particle diameters 1-25 microns

Яндекс.Метрика